Abstract Mitochondria are inherited exclusively from the mothers and are required for the proper development of embryos.Hence, germline mitochondrial quality is Time and Space in Digital Game Storytelling highly regulated during oogenesis to ensure oocyte viability.How nutrient availability influences germline mitochondrial quality control is unclear.Here we find that fasting leads to the accumulation of mitochondrial clumps and oogenesis arrest in Drosophila.Fasting induces the downregulation of the DIP1-Clueless pathway, leading to an increase in the expression of a stable intronic sequence RNA called sisR-1.
Mechanistically, sisR-1 localizes to the mitochondrial clumps to inhibit the poly-ubiquitination of the outer mitochondrial protein Porin/VDAC1, thereby suppressing p62-mediated mitophagy.Alleviation of the “Don’t Bring Me a Dog…I’ll Just Keep It”: Understanding Unplanned Dog Acquisitions Amongst a Sample of Dog Owners Attending Canine Health and Welfare Community Events in the United Kingdom fasting-induced high sisR-1 levels by either sisR-1 RNAi or refeeding leads to mitophagy, the resumption of oogenesis and an improvement in oocyte quality.Thus, our study provides a possible mechanism by which fasting can improve oocyte quality by modulating the mitochondrial quality control pathway.Of note, we uncover that the sisR-1 response also regulates mitochondrial clumping and oogenesis during protein deprivation, heat shock and aging, suggesting a broader role for this mechanism in germline mitochondrial quality control.